His Tag Monoclonal Antibody, IgG1, Clone: [2F4B5], Unconjugated, Mouse

Product Overview

• Article Name: His Tag Monoclonal Antibody, IgG1, Clone: [2F4B5], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA000011M1M
• Supplier Catalog Number: CSB-MA000011M1m
• Alternative Catalog Number: CSB-MA000011M1M-100UL, CSB-MA000011M1M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IP, WB
• Species Reactivity: All
• Conjugation: Unconjugated
• Alternative Names: His Tag

Product Properties

• Clonality: Monoclonal
• Clone Designation: [2F4B5]
• Isotype: IgG1
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: His Tag

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:640000, IP:2µl-5µl

Images

Immunoprecipitating His in 293 whole cell lysate transfected with His
Lane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His.Lane 2: 2F4B5 antibody(2µg) + 293 whole cell lysate transfected with His (500µg)
Lane 3: 293 whole cell lysate transfected with His (5µg)
For western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000

Western Blot
Positive WB detected in: 200ng recombinant protein with His tagHis antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4 KDa
Observed band size: 43.4 KDa
Exposure time:5min

Western Blot
Positive WB detected in: 200ng recombinant protein with His tagHis antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size:62.5 KDa
Observed band size: 66 KDa
Exposure time:5min

Western Blot
Positive WB detected in:200ng of four Recombinant protein with his tagAll lanes: His antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 43.4, 62.5,56,80.6 KDa
Observed band size:43.4, 66, 57 ,90 KDa
Exposure time:5min