Flag Tag Monoclonal Antibody, IgG2a, Clone: [4G5C8], Unconjugated, Mouse

Product Overview

• Article Name: Flag Tag Monoclonal Antibody, IgG2a, Clone: [4G5C8], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA000021M0M
• Supplier Catalog Number: CSB-MA000021M0m
• Alternative Catalog Number: CSB-MA000021M0M-100UL, CSB-MA000021M0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IP, WB
• Species Reactivity: All
• Conjugation: Unconjugated
• Alternative Names: Flag Tag

Product Properties

• Clonality: Monoclonal
• Clone Designation: [4G5C8]
• Isotype: IgG2a
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: Flag Tag

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:10000, IF:1:100-1:300, IP:0.5µl-2µl

Images

CSB-MA000021M0m

Western Blot
Positive WB detected in: 10ng Flag Tag fusion proteinFlag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng
All lanes: Flag Tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: Flag Tag fusion protein1, 2, 3, 4 at 50ng
All lanes: Flag Tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 59.6, 36, 35, 42 kDa
Observed band size: 59.6, 36, 35, 42 kDa

Western Blot
Positive WB detected in: Flag Tag fusion protein at 10ng
All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng
All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: 5 different recombinant proteins with Flag Tag
All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution