GST Tag Monoclonal Antibody, IgG2b, Clone: [3D3E4], Unconjugated, Mouse
Catalog Number:
CSB-MA000031M1M
- Images (7)
| Article Name: | GST Tag Monoclonal Antibody, IgG2b, Clone: [3D3E4], Unconjugated, Mouse |
| Biozol Catalog Number: | CSB-MA000031M1M |
| Supplier Catalog Number: | CSB-MA000031M1m |
| Alternative Catalog Number: | CSB-MA000031M1M-100UL, CSB-MA000031M1M-50UL |
| Manufacturer: | Cusabio |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, IP, WB |
| Species Reactivity: | All |
| Conjugation: | Unconjugated |
| Alternative Names: | GST Tag |
| Clonality: | Monoclonal |
| Clone Designation: | [3D3E4] |
| Isotype: | IgG2b |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein A purified |
| Form: | Liquid |
| Target: | GST Tag |
| Application Dilute: | Recommended dilution: WB:1:10000-1:640000, IF:1:100-1:300, FC: 1:100-1:300, IP: 1ul-5ul |
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CSB-MA000031M1m |
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Immunofluorescence staining of 293F transfected cells with CSB-MA000031M1m at 1:94.5, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4掳C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). |
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Immunoprecipitating GST in transfected-293F whole cell lysate Lane 1: Mouse control IgG instead of CSB-MA000031M1m in 293F cell lysate Transfected with GST Lane 2: CSB-MA000031M1m (5碌g) + Transfected-293F whole cell lysate (500碌g) Lane 3: Transfected-293F whole cell lysate (10碌g) For western blotting, the blot was detected with CSB-MA000031M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000 |
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Western Blot Positive WB detected in: 25ng Recombinant protein with GST tag from E.coli All lanes: GST antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32.6, 30.1, 29.5, 37.4, 30.4, 60.5, 38.1, 61.6 KDa Observed band size: 31, 30, 31, 37, 30, 60, 37, 61 KDa Exposure time: 5min |
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Western Blot Positive WB detected in: 50ng Recombinant protein with GST tag from E.coli All lanes: GST antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 26, 102.9, 75.6 KDa Observed band size: 26, 103, 76 KDa Exposure time: 5min |
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Western Blot Positive WB detected in: Recombinant protein with GST tag from E.coli at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng All lanes: GST antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa Exposure time: 5min |
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Western Blot Positive WB detected in: 25ng recombinant protein with GST tag from E.coli GST antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 26 KDa Observed band size: 26 KDa Exposure time: 5min |
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