GFP Tag Monoclonal Antibody, IgG2b, Clone: [6C11C11], Unconjugated, Mouse
Catalog Number:
CSB-MA000051M1M
- Images (7)
| Article Name: | GFP Tag Monoclonal Antibody, IgG2b, Clone: [6C11C11], Unconjugated, Mouse |
| Biozol Catalog Number: | CSB-MA000051M1M |
| Supplier Catalog Number: | CSB-MA000051M1m |
| Alternative Catalog Number: | CSB-MA000051M1M-100UG, CSB-MA000051M1M-50UG |
| Manufacturer: | Cusabio |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, IP, WB |
| Species Reactivity: | All |
| Conjugation: | Unconjugated |
| Alternative Names: | GFP Tag |
| Clonality: | Monoclonal |
| Clone Designation: | [6C11C11] |
| Isotype: | IgG2b |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | GFP Tag |
| Application Dilute: | Recommended dilution: WB:1:50000-1:6400000, IF:1:50-1:200, FC:1:100-1:300, IP:1µl-2µl |
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Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4°C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106 cells) for 1 h at 4C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4C. |
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Overlay histogram showing 293F cells untransfected (green line) or transfected with GFP (red line) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4°C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106 cells) for 1 h at 4C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4C. |
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Immunofluorescence staining of 293F cells transfected with GFP with CSB-MA000051M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L). |
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CSB-MA000051M1m |
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Western Blot Positive WB detected in: 50ng recombinant protein All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time:5min |
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Western Blot Positive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ng All lanes: GFP antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 32 KDa Observed band size: 32 KDa Exposure time:5min |
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Western Blot Positive WB detected in: 1,2,3 and 4 are Recombinant proteins with GFP tag for 50ng, 5, 293F whole cell lysate transfected with GFP for 5ug All lanes GFP antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively Observed band size: 32,32,50,32,32 KDa Exposure time:1min |
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