GAPDH Monoclonal Antibody, IgG1, Clone: [14C2F11], Unconjugated, Mouse

Product Overview

• Article Name: GAPDH Monoclonal Antibody, IgG1, Clone: [14C2F11], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA000071M0M
• Supplier Catalog Number: CSB-MA000071M0m
• Alternative Catalog Number: CSB-MA000071M0M-100UL, CSB-MA000071M0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, IHC, IP, WB
• Species Reactivity: Human, Rabbit, Rat
• Conjugation: Unconjugated
• Alternative Names: GAPDH

Product Properties

• Clonality: Monoclonal
• Clone Designation: [14C2F11]
• Isotype: IgG1
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: GAPDH

Application Notes

• Application Dilute: Recommended dilution: WB:1:5000-1:1600000, IHC:1:50-1:500, IF:1:50-1:200, IP:1µl-2µl, FC:1:100-1:300

Images

CSB-MA000071M0m

Western Blot
Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time:30S

Western Blot
Positive WB detected in: Rat heart tissue, Rat kidney tissue, Rat skeletal muscle tissue, Rat liver tissue, Rat brain tissue tissue, Rat spleen tissue
All lanes GAPDH antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time:1min

Western Blot
Positive WB detected in: Rabbit heart tissue, Rabbit liver tissue,Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time:5min

Western Blot
Positive WB detected in: 15ug hela whole cell lysate
GAPDH antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min

Western Blot
Positive WB detected in: Hela whole cell lysate at 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ug
All lanes: GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 5min

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: GAPDH antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time: 30s