GAPDH Monoclonal Antibody, IgG1, Clone: [10B4E3], Unconjugated, Mouse
Catalog Number:
CSB-MA000071M1M
- Images (7)
| Article Name: | GAPDH Monoclonal Antibody, IgG1, Clone: [10B4E3], Unconjugated, Mouse |
| Biozol Catalog Number: | CSB-MA000071M1M |
| Supplier Catalog Number: | CSB-MA000071M1m |
| Alternative Catalog Number: | CSB-MA000071M1M-100UL, CSB-MA000071M1M-50UL |
| Manufacturer: | Cusabio |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Rabbit |
| Conjugation: | Unconjugated |
| Alternative Names: | GAPDH |
| Clonality: | Monoclonal |
| Clone Designation: | [10B4E3] |
| Isotype: | IgG1 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | GAPDH |
| Application Dilute: | Recommended dilution: WB: 1:5000-1:80000, IHC: 1:200-1:500, IF: 1:50-1:100, IP: 2µl-8µl |
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IHC image of CSB-MA000071M1m diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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CSB-MA000071M1m |
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Western Blot Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate, All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 10s |
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Western Blot Positive WB detected in: SP2/0 whole cell lysate, NIH/3T3 whole cell lysate, Raw264.7 whole cell lysate, Mouse Heart tissue, Mouse lung tissue,Mouse kidney tissue All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 10s |
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Western Blot Positive WB detected in: Rabbit heart tissue, Rabbit kidney tissue, Rabbit muscle tissue All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 10s |
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Western Blot Positive WB detected in: Hela whole cell lysate at 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug All lanes:GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 10S |
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Western Blot Positive WB detected in: 15ug hela whole cell lysate GAPDH antibody at 1:10000, 1:20000, 1:40000, 1:80000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 10S |
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