GAPDH Monoclonal Antibody, IgG1, Clone: [10H4D6], Unconjugated, Mouse

Product Overview

• Article Name: GAPDH Monoclonal Antibody, IgG1, Clone: [10H4D6], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA000071M2M
• Supplier Catalog Number: CSB-MA000071M2m
• Alternative Catalog Number: CSB-MA000071M2M-100UL, CSB-MA000071M2M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rabbit, Rat
• Conjugation: Unconjugated
• Alternative Names: GAPDH

Product Properties

• Clonality: Monoclonal
• Clone Designation: [10H4D6]
• Isotype: IgG1
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: GAPDH

Application Notes

• Application Dilute: Recommended dilution: WB: 1:5000-1:80000, IHC: 1:200-1:500, IF: 1:50-1:100, IP: 2µl-8µl

Images

CSB-MA000071M2m

Western Blot
Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MG-63 whole cell lysate, U251 whole cell lysate, SH-SY5Y whole cell lysate
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time:5min

Western Blot
Positive WB detected in: SP2/0 whole cell lysate, NIH/3T3 whole cell lysate, Raw264.7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time:5min

Western Blot
Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat spleen tissue, Rat brain tissue, Rat skeletal tissue, Rat stomach tissue, Rat kidney tissue
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time:10S

Western Blot
Positive WB detected in: Rabbit heart tissue, Rabbit kidney tissue, Rabbit skeletal muscle tissue
All lanes GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time:5min

Western Blot
Positive WB detected in: Hela whole cell lysate at 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ug, 0.15625ug, 0.078ugAll lanes:GAPDH antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure time:5min

Western Blot
Positive WB detected in: 15ug hela whole cell lysate GAPDH antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 36 KDa
Observed band size: 36 KDa
Exposure timeL: 5min