ACTB Monoclonal Antibody, IgG2b, Clone: [1D12E12B5], Unconjugated, Mouse

Catalog Number: CSB-MA000091M1M
Article Name: ACTB Monoclonal Antibody, IgG2b, Clone: [1D12E12B5], Unconjugated, Mouse
Biozol Catalog Number: CSB-MA000091M1M
Supplier Catalog Number: CSB-MA000091M1m
Alternative Catalog Number: CSB-MA000091M1M-100UL, CSB-MA000091M1M-50UL
Manufacturer: Cusabio
Host: Mouse
Category: Antikörper
Application: ELISA, FC, IF, IHC, WB
Species Reactivity: Human, Mouse, Rabbit, Rat
Conjugation: Unconjugated
Alternative Names: ACTB
Clonality: Monoclonal
Clone Designation: [1D12E12B5]
Isotype: IgG2b
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein A purified
Form: Liquid
Target: ACTB
Application Dilute: Recommended dilution: WB: 1:5000-1:80000, IHC: 1:500-1:1000, IF: 1:50-1:200, FC: 1:100-1:300
Western Blot
Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Western Blot
Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, Rabbit kidney tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Western Blot
Positive WB detected in: Mouse liver tissue, Mouse brain tissue, Mouse lung tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse stomach tissue
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Western Blot
Positive WB detected in: Rabbit liver tissue, Rabbit brain tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit stomach tissue, A549 whole cell lysate
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
Western Blot
Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, U87 whole cell lysate
All lanes: ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min
CSB-MA000091M1m
Western Blot
Positive WB detected in: 293T whole cell lysate at 40ug, 20ug, 10ug
All lanes:ACTB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
Exposure time: 5min