HA Tag Monoclonal Antibody, IgG2b, Clone: [18B11H6], Unconjugated, Mouse

Product Overview

• Article Name: HA Tag Monoclonal Antibody, IgG2b, Clone: [18B11H6], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA000141M0M
• Supplier Catalog Number: CSB-MA000141M0m
• Alternative Catalog Number: CSB-MA000141M0M-100UL, CSB-MA000141M0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, IP, WB
• Species Reactivity: All
• Conjugation: Unconjugated
• Alternative Names: HA Tag

Product Properties

• Clonality: Monoclonal
• Clone Designation: [18B11H6]
• Isotype: IgG2b
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein A purified
• Form: Liquid
• Target: HA Tag

Application Notes

• Application Dilute: Recommended dilution: WB:1:5000-1:160000, IF:1:50-1:200, IP: 1:5000-1:100000, FC:1:50-1:200

Images

Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

CSB-MA000141M0m

Western Blot
Positive WB detected in: 3 different overexpression lysates with HA tagged
All lanes: HA Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 35, 48 kDa
Observed band size: 35, 35, 48 kDa

Western Blot
Positive WB detected in: HA Tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
All lanes: HA Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: HA Tagged fusion protein
All lanes: HA Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Western Blot
Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
All lanes: HA Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution