E Tag Monoclonal Antibody, IgG1, Clone: [11H12B3], Unconjugated, Mouse
Catalog Number:
CSB-MA000151M0M
- Images (5)
| Article Name: | E Tag Monoclonal Antibody, IgG1, Clone: [11H12B3], Unconjugated, Mouse |
| Biozol Catalog Number: | CSB-MA000151M0M |
| Supplier Catalog Number: | CSB-MA000151M0m |
| Alternative Catalog Number: | CSB-MA000151M0M-100UL, CSB-MA000151M0M-50UL |
| Manufacturer: | Cusabio |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, IP, WB |
| Species Reactivity: | All |
| Conjugation: | Unconjugated |
| Alternative Names: | E Tag |
| Clonality: | Monoclonal |
| Clone Designation: | [11H12B3] |
| Isotype: | IgG1 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | E Tag |
| Application Dilute: | Recommended dilution: ELISA:1:4000-1:8000, WB:1:1000-1:320000, IP:1µg-5µg |
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ELISA E Tag antibody in E Tagged fusion protein and Control protein(no E Tag) Antigen at dilution of 1ug/ml E Tag antibody at (0-0.15ug) Goat polyclonal to Mouse IgG at 1/50000 dilution |
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Immunoprecipitating E Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg) Lane 3: 293F transfected whole cell lysate (20µg) |
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Western Blot Positive WB detected in: E Tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: E Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa |
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Western Blot Positive WB detected in: E Tagged fusion protein All lanes: E Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa |
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Western Blot Positive WB detected in: 1-3 lanes: E-tagged fusion protein, 4-5 lanes: Recombinant protein without E Tagged, 6 lane: BSA-E Tag All lanes: E Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 30, 68, 55, 67-157kDa Observed band size: 30, 68, 55, 67-157kDa |
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