V5 Tag Monoclonal Antibody, IgG1, Clone: [8A7A8], Unconjugated, Mouse

Product Overview

• Article Name: V5 Tag Monoclonal Antibody, IgG1, Clone: [8A7A8], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA000161M0M
• Supplier Catalog Number: CSB-MA000161M0m
• Alternative Catalog Number: CSB-MA000161M0M-100UL, CSB-MA000161M0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, IP, WB
• Species Reactivity: All
• Conjugation: Unconjugated
• Alternative Names: V5 Tag

Product Properties

• Clonality: Monoclonal
• Clone Designation: [8A7A8]
• Isotype: IgG1
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: V5 Tag

Application Notes

• Application Dilute: Recommended dilution: WB: 1:2000-1:80000, IF: 1:100-1:200, IP: 2µl-5µl, FC: 1:100-1:300

Images

CSB-MA000161M0m

Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).

Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
Lane 1: Mouse control IgG1 instead of CSB-MA000161M0m in Transfected HEK293F cells whole cell lysate
Lane 2: CSB-MA000161M0m (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg)
Lane 3: Transfected HEK293F cells whole cell lysate (20µg)
For western blotting, the blot was detected with CSB-MA000161M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000

Western Blot
Positive WB detected in: 50ng V5 Tagged fusion protein
V5 Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 37.8 KDa
Observed band size: 43 KDa
Exposure time: 10S

Western Blot
Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:5000
Lane 1: Recombinant V5 Tagged fusion protein at 50ng
Lane 2: Recombinant V5 Tagged fusion protein at 25ng
Lane 3: Recombinant V5 Tagged fusion protein at 12.25ng
Lane 4: Recombinant V5 Tagged fusion protein at 6.25ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 37.8 kd
Observed band size: 43 kd
Exposure time: 5min

Western Blot
Positive WB detected in: V5 Tagged fusion protein 1, 2, 3, 4 at 50ng
All lanes: V5 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50.2, 47.6, 66.7, 37.8 KDa
Observed band size: 52, 47, 67, 42 KDa
Exposure time: 30s

Western Blot
Positive WB detected in: V5 Tagged fusion protein 1, 2, 3, 4, at 50ng
All lanes: V5 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31.9, 20.4, 24.2, 53.4 KDa
Observed band size: 32, 21, 28, 58 KDa
Exposure time: 5min