CD44 Monoclonal Antibody, Clone: [6G2D2], Unconjugated, Mouse

Product Overview

• Article Name: CD44 Monoclonal Antibody, Clone: [6G2D2], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA004938A0M
• Supplier Catalog Number: CSB-MA004938A0m
• Alternative Catalog Number: CSB-MA004938A0M-100UL, CSB-MA004938A0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I, CD44, CD44, LHR, MDU2, MDU3, MIC4

Product Properties

• Clonality: Monoclonal
• Clone Designation: [6G2D2]
• UniProt: P16070
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: CD44

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:40000, IHC:1:50-1:200, IF:1:50-1:200

Images

Overlay histogram showing Hela cells stained with CSB-MA004938A0m (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of Hela cells with CSB-MA004938A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of MCF-7 cells with CSB-MA004938A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA004938A0m diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Hela whole cell lysate, HUVEC whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate
All lanes: CD44 antibody at 1:1500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa
Observed band size: 95 kDa

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: CD44 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 82, 4, 78, 77, 81, 79, 75, 54,47, 40, 44, 33, 74, 76, 38, 16 kDa
Observed band size: 95 kDa