CD48 Monoclonal Antibody, IgG1, Clone: [6F9H2], Unconjugated, Mouse

Product Overview

• Article Name: CD48 Monoclonal Antibody, IgG1, Clone: [6F9H2], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA004941A0M
• Supplier Catalog Number: CSB-MA004941A0m
• Alternative Catalog Number: CSB-MA004941A0M-100UL, CSB-MA004941A0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: Antigen CD48 antibody, B cell membrane protein antibody, B lymphocyte activation marker BLAST 1 antibody, B-cell activation marker antibody, B-lymphocyte activation marker BLAST-1 antibody, BCM 1 surface antigen antibody, BCM1 antibody, BCM1 surface antigen antibody, BLAST 1 antibody, BLAST antibody, BLAST1 antibody, CD 48 antibody, CD48 antibody, CD48 antigen (B cell membrane protein) antibody, CD48 antigen antibody, CD48 molecule antibody, CD48 protein antibody, CD48_HUMAN antibody, hCD48 antibody, Leucocyte antigen MEM 102 antibody, Leukocyte antigen MEM-102 antibody, mCD48 antibody, MEM 102 antibody, MEM-102 antibody, MEM102 antibody, Signaling lymphocytic activation molecule 2 antibody, SLAM family member 2 antibody, SLAMF 2 antibody, SLAMF2 antibody, TCT.1 antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [6F9H2]
• Isotype: IgG1
• UniProt: P09326
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: CD48

Application Notes

• Application Dilute: Recommended dilution: WB: 1:2000-1:128000, IF: 1:50-1:200, FC: 1:100-1:500

Images

Overlay Peak curve showing JK cells stained with CSB-MA004941A0m (red line) at 1:200. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106 cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of JK cells with CSB-MA004941A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Western Blot
Positive WB detected in: Jurkat whole cell lysate, Raji whole cell lysate, Daudi whole cell lysate, Mouse liver tissue
All lanes: CD48 antibody at 1:4000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 28, 20KDa
Observed band size: 43 KDa
Exposure time:5min

Western Blot
Positive WB detected in: 20ug Raji whole cell lysate
CD48 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 28, 20 KDa
Observed band size: 43 KDa
Exposure time:5min