CDH1 Monoclonal Antibody, IgG1, Clone: [9H2D3], Unconjugated, Mouse

Product Overview

• Article Name: CDH1 Monoclonal Antibody, IgG1, Clone: [9H2D3], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA005034A0M
• Supplier Catalog Number: CSB-MA005034A0m
• Alternative Catalog Number: CSB-MA005034A0M-100UL, CSB-MA005034A0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: Cadherin-1 (CAM 120/80) (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3], CDH1, CDHE UVO

Product Properties

• Clonality: Monoclonal
• Clone Designation: [9H2D3]
• Isotype: IgG1
• UniProt: P12830
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: CDH1

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200

Images

Overlay histogram showing MCF-7 cells stained with CSB-MA005034A0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of Hela cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of MCF-7 cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of SW620 cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Western Blot
Positive WB detected in: Caco205 whole cell lysate
All lanes: CDH1 antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 98, 91 kDa
Observed band size: 110, 120 kDa