FLOT1 Monoclonal Antibody, IgG2a, Clone: [4C8B6], Unconjugated, Mouse

Product Overview

• Article Name: FLOT1 Monoclonal Antibody, IgG2a, Clone: [4C8B6], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA008727A0M
• Supplier Catalog Number: CSB-MA008727A0m
• Alternative Catalog Number: CSB-MA008727A0M-100UL, CSB-MA008727A0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: FLOT 1 antibody, FLOT1 antibody, FLOT1_HUMAN antibody, Flotillin-1 antibody, Flotillin1 antibody, Integral membrane component of caveolae antibody, Reggie 2 antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [4C8B6]
• Isotype: IgG2a
• UniProt: O75955
• Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20C, Avoid freeze / thaw cycles.
• Purity: >95%, Protein A purified
• Form: Liquid
• Target: FLOT1

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Images

Overlay Peak curve showing Hela cells stained with CSB-MA008727A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106 cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of Hela cells with CSB-MA008727A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

IHC image of CSB-MA008727A0m diluted at 1:100 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA008727A0m diluted at 1:100 and staining in paraffin-embedded human cervical cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, 3T3 whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue
All lanes: FLOT1 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 48, 43 kDa
Observed band size: 48 KDa
Exposure time:5min