MAGEA4 Monoclonal Antibody, IgG1, Clone: [1G10H5], Unconjugated, Mouse

Product Overview

• Article Name: MAGEA4 Monoclonal Antibody, IgG1, Clone: [1G10H5], Unconjugated, Mouse
• Biozol Catalog Number: CSB-MA013330A0M
• Supplier Catalog Number: CSB-MA013330A0m
• Alternative Catalog Number: CSB-MA013330A0M-100UL, CSB-MA013330A0M-50UL
• Manufacturer: Cusabio
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FC, IHC
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: Cancer/testis antigen 1.4 antibody, CT1.4 antibody, MAGA4_HUMAN antibody, MAGE 4 antibody, MAGE 4 antigen antibody, MAGE 41 antibody, MAGE 41 antigen antibody, MAGE 4A antibody, MAGE 4B antibody, MAGE X2 antibody, MAGE X2 antigen antibody, MAGE-4 antigen antibody, MAGE-41 antigen antibody, MAGE-X2 antigen antibody, MAGE4 antibody, MAGE41 antibody, MAGE4A antibody, MAGE4B antibody, MAGEA 4 antibody, MAGEA4 antibody, Melanoma antigen family A 4 antibody, Melanoma associated antigen 4 antibody, Melanoma-associated antigen 4 antibody, MGC21336 antibody

Product Properties

• Clonality: Monoclonal
• Clone Designation: [1G10H5]
• Isotype: IgG1
• UniProt: P43358
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: MAGEA4

Application Notes

• Application Dilute: Recommended dilution: IHC:1:200-1:500, FC:1:50-1:200

Images

Overlay Peak curve showing A375 cells stained with CSB-MA013331A0m (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of CSB-MA013330A0m diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-MA013330A0m diluted at 1:200 and staining in paraffin-embedded human bladder cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-MA013330A0m diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-MA013330A0m diluted at 1:200 and staining in paraffin-embedded human gastric cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.