CD146 Monoclonal Antibody, IgG2a, Clone: [4D10C7], Unconjugated, Mouse
Catalog Number:
CSB-MA013563A0M
- Images (7)
| Article Name: | CD146 Monoclonal Antibody, IgG2a, Clone: [4D10C7], Unconjugated, Mouse |
| Biozol Catalog Number: | CSB-MA013563A0M |
| Supplier Catalog Number: | CSB-MA013563A0m |
| Alternative Catalog Number: | CSB-MA013563A0M-100UL, CSB-MA013563A0M-50UL |
| Manufacturer: | Cusabio |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | Cell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146), MCAM, MUC18 |
| Clonality: | Monoclonal |
| Clone Designation: | [4D10C7] |
| Isotype: | IgG2a |
| UniProt: | P43121 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | CD146 |
| Application Dilute: | Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200 |
|
|
Overlay histogram showing MCF-7 cells stained with CSB-MA013563A0m (red line) at 1:400. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. |
|
|
Immunofluorescence staining of A375 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). |
|
|
Immunofluorescence staining of Hela cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). |
|
|
Immunofluorescence staining of MCF-7 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). |
|
|
Immunofluorescence staining of THP-1 cells with CSB-MA013563A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). |
|
|
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CD146 antibody at 1:1250 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa |
|
|
Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate All lanes: CD146 antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa |
Product Guarantee and Expert Support
