ALDH5A1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ALDH5A1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA001577LA01HU
• Supplier Catalog Number: CSB-PA001577LA01HU
• Alternative Catalog Number: CSB-PA001577LA01HU-100UG, CSB-PA001577LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: Aldedehyde dehydrogenase 5 family antibody, Aldehyde dehydrogenase 5 family member A1 antibody, Aldehyde dehydrogenase 5A1 antibody, Aldehyde dehydrogenase family 5 member A1 antibody, ALDH5A 1 antibody, Aldh5a1 antibody, mitochondrial antibody, Mitochondrial succinate semialdehyde dehydrogenase antibody, NAD(+) dependent succinic semialdehyde dehydrogenase antibody, NAD(+)-dependent succinic semialdehyde dehydrogenase antibody, SSADH antibody, SSDH antibody, SSDH_HUMAN antibody, Succinate semialdehyde dehydrogenase antibody, Succinate-semialdehyde dehydrogenase antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P51649
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: ALDH5A1

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Images

IHC image of CSB-PA001577LA01HU diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Rat heart tissue, Rat brain tissue, Mouse liver tissue
All lanes: ALDH5A1 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58, 59 kDa
Observed band size: 58 kDa

Immunofluorescence staining of HepG2 cells with CSB-PA001577LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA001577LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA001577LA01HU at dilution of 1:100