ATP5F1A Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ATP5F1A Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA002344HA01HU
• Supplier Catalog Number: CSB-PA002344HA01HU
• Alternative Catalog Number: CSB-PA002344HA01HU-100UG, CSB-PA002344HA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: ATP synthase alpha chain antibody, ATP synthase alpha chain, mitochondrial antibody, ATP synthase subunit alpha antibody, ATP synthase subunit alpha mitochondrial antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody, ATP sythase (F1 ATPase) alpha subunit antibody, ATP5A antibody, Atp5a1 antibody, ATP5AL2 antibody, ATPA_HUMAN antibody, ATPM antibody, Epididymis secretory sperm binding protein Li 123m antibody, hATP1 antibody, HEL-S-123m antibody, MC5DN4 antibody, mitochondrial antibody, Mitochondrial ATP synthetase antibody, Mitochondrial ATP synthetase oligomycin resistant antibody, Modifier of Min 2 antibody, Modifier of Min 2 mouse homolog antibody, Modifier of Min 2, mouse, homolog of antibody, MOM2 antibody, OMR antibody, ORM antibody, OTTHUMP00000163475 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P25705
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: ATP5F1A

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000

Images

Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA002344HA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA002344HA01HU at dilution of 1:100

Western Blot
Positive WB detected in: K562 whole cell lysate, Mouse heart tissue
All lanes: ATP5F1A antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa

Western Blot
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Mouse brain tissue, Rat brain tissue
All lanes: ATP5A1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa

Immunoprecipitating ATP5A1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1?g) instead of CSB-PA002344HA01HU in Hela whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA002344HA01HU (4?g) + Hela whole cell lysate (500?g)
Lane 3: Hela whole cell lysate (20?g)