BDH1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: BDH1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA002648LA01HU
• Supplier Catalog Number: CSB-PA002648LA01HU
• Alternative Catalog Number: CSB-PA002648LA01HU-100UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: 3 hydroxybutyrate dehydrogenase (heart, mitochondrial) antibody, 3-hydroxybutyrate dehydrogenase antibody, BDH antibody, BDH_HUMAN antibody, BDH1 antibody, D beta hydroxybutyrate dehydrogenase, mitochondrial antibody, D-beta-hydroxybutyrate dehydrogenase antibody, mitochondrial antibody

Product Properties

• Clonality: Polyclonal
• UniProt: Q02338
• Buffer: Preservative: 0.02% sodium azide<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: BDH1

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:3000, IHC:1:100-1:300, IF:1:20-1:100

Images

Immunofluorescence staining of MCF-7 cell with CSB-PA002648LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cell with CSB-PA002648LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA002648LA01HU diluted at 1:150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA002648LA01HU diluted at 1:150 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in:Mouse Heart tissue lysate, Mouse Liver tissue lysate, Mouse Brain tissue lysate, Rat Heart tissue lysate, Rat Livert tissue lysate, Rat Brain tissue lysate, MCF7 whole cell lysate
All lanes: BDH1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39, 42 kDa
Observed band size: 39 kDa