GPI Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GPI Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA00367A0RB
• Supplier Catalog Number: CSB-PA00367A0Rb
• Alternative Catalog Number: CSB-PA00367A0RB-100UG, CSB-PA00367A0RB-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: AMF antibody, Aurocrine motility factor antibody, Autocrine motility factor antibody, DKFZp686C13233 antibody, EC 5.3.1.9 antibody, G6PI_HUMAN antibody, Glucose phosphate isomerase antibody, Glucose-6-phosphate isomerase antibody, GNPI antibody, GPI antibody, Gpi1 antibody, Hexose monophosphate isomerase antibody, Hexosephosphate isomerase antibody, Neuroleukin antibody, NLK antibody, Oxoisomerase antibody, PGI antibody, PHI antibody, Phosphoglucose isomerase antibody, Phosphohexomutase antibody, Phosphohexose isomerase antibody, Phosphosaccharomutase antibody, SA 36 antibody, SA-36 antibody, SA36 antibody, Sperm antigen 36 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P06744
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: GPI

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Images

Immunofluorescent analysis of PC-3 cells using CSB-PA00367A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00367A0Rb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA00367A0Rb at dilution of 1:100

Immunoprecipitating GPI in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA00367A0Rb in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA00367A0Rb (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)

Western Blot
Positive WB detected in: PC-3 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue, Mouse brain tissue
All lanes: GPI antibody at 2.7µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64, 65 kDa
Observed band size: 64 kDa