CA2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CA2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA004370HA01HU
• Supplier Catalog Number: CSB-PA004370HA01HU
• Alternative Catalog Number: CSB-PA004370HA01HU-100UG, CSB-PA004370HA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: CA 2 antibody, CA II antibody, CA-II antibody, Ca2 antibody, CAC antibody, CAH2_HUMAN antibody, CAII antibody, Car 2 antibody, Car2 antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase C, formerly antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, epididymis luminal protein 76 antibody, Epididymis secretory protein Li 282 antibody, HEL-76 antibody, HEL-S-282 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P00918
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: CA2

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200

Images

Immunofluorescent analysis of Hela cells using CSB-PA004370HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA004370HA01HU at dilution of 1:100

Western Blot
Positive WB detected in: Rat kidney tissue, Rat heart tissue, Rat liver tissue, Mouse lung tissue, Mouse kidney tissue
All lanes: CA2 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa

IHC image of CSB-PA004370HA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA004370HA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.