Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA00465A0Rb at dilution of 1:100
Western blot All lanes: YARS antibody at 2µg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
Immunofluorescence staining of HepG2 cells with CSB-PA00465A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of SH-SY5Y cells with CSB-PA00465A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA00465A0Rb at dilution of 1:100
* VAT and and shipping costs not included. Errors and price changes excepted