CCND2 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA004812ESR2HU
- Images (6)
| Article Name: | CCND2 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA004812ESR2HU |
| Supplier Catalog Number: | CSB-PA004812ESR2HU |
| Alternative Catalog Number: | CSB-PA004812ESR2HU-100UL, CSB-PA004812ESR2HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, WB |
| Species Reactivity: | Human, Mouse |
| Conjugation: | Unconjugated |
| Alternative Names: | CCND 2 antibody, ccnd2 antibody, CCND2_HUMAN antibody, CyclinD2 antibody, G1/S specific cyclin D2 antibody, G1/S-specific cyclin-D2 antibody, KIAK0002 antibody, MGC102758 antibody, MPPH3 antibody |
| Clonality: | Polyclonal |
| UniProt: | P30279 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | CCND2 |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:500, IF:1:50-1:200 |
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Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA004812ESR2HU at dilution of 1:100 |
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA004812ESR2HU at dilution of 1:100 |
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Immunofluorescence staining of HepG2 cells with CSB-PA004812ESR2HU at 1:75, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA004812ESR2HU diluted at 1:225 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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IHC image of CSB-PA004812ESR2HU diluted at 1:225 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: CCND2 antibody at 2.25ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa |
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