CSNK1D Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CSNK1D Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA006067LA01HU
• Supplier Catalog Number: CSB-PA006067LA01HU
• Alternative Catalog Number: CSB-PA006067LA01HU-100UG, CSB-PA006067LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: Casein Kinase 1 delta antibody, Casein kinase I delta isoform antibody, Casein kinase I isoform delta antibody, CKI delta antibody, CKI-delta antibody, CKId antibody, CKIdelta antibody, CSNK 1D antibody, CSNK1D antibody, HCKID antibody, KC1D_HUMAN antibody, Protein kinase CK1 delta antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P48730
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: CSNK1D

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500, IP:1:200-1:2000

Images

Immunofluorescence staining of HepG2 cells with CSB-PA006067LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA006067LA01HU diluted at 1:1000 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating CSNK1D in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA006067LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA006067LA01HU (6µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)

Western Blot
Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, 293T whole cell lysate, Rat brain tissue, Mouse liver tissue
All lanes: CSNK1D antibody at 5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 47 kDa
Observed band size: 48 kDa

IHC image of CSB-PA006067LA01HU diluted at 1:1000 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA006067LA01HU diluted at 1:1000 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.