CYP7A1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: CYP7A1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA006460LA01HU
• Supplier Catalog Number: CSB-PA006460LA01HU
• Alternative Catalog Number: CSB-PA006460LA01HU-100UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: Cholesterol 7 alpha hydroxylase antibody, Cholesterol 7 alpha monooxygenase antibody, Cholesterol 7-alpha-hydroxylase antibody, Cholesterol 7-alpha-monooxygenase antibody, CP7A antibody, CP7A1_HUMAN antibody, CYP 7 antibody, CYP7 antibody, CYP7A1 antibody, CYPVII antibody, Cytochrome P450 7A1 antibody, Cytochrome P450, family 7, subfamily A, polypeptide 1 antibody, Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody, Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody, MGC126826 antibody, MGC138389 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P22680
• Buffer: Preservative: 0.02% sodium azideConstituents: PBS containing 50% glycerol
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: CYP7A1

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000 , IHC:1:100-1:300

Images

IHC image of CSB-PA006460LA01HU diluted at 1:150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA006460LA01HU diluted at 1:150 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate
All lanes:CYP7A1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size:58 kDa
Observed band size:58 kDa