DDHD2 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: DDHD2 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA006586LA01HU
• Supplier Catalog Number: CSB-PA006586LA01HU
• Alternative Catalog Number: CSB-PA006586LA01HU-100UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Rat
• Conjugation: Unconjugated
• Alternative Names: DDHD domain containing protein 2 antibody, DDHD domain-containing protein 2 antibody, Ddhd2 antibody, DDHD2_HUMAN antibody, KIAA0725 antibody, mKIAA0725 antibody, Phospholipase DDHD2 antibody, SAM antibody, SAM, WWE and DDHD domain containing protein 1 antibody, SAMWD1 antibody, WWE and DDHD domain-containing protein 1 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: O94830
• Buffer: Preservative: 0.02% sodium azide<br />Constituents: PBS containing 50% glycerol
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: DDHD2

Application Notes

• Application Dilute: Recommended dilution: WB: 1:1000-1:5000, IHC: 1:50-1:500, IF:1:20-1:200

Images

Immunohistochemistry of

Immunofluorescence staining of Hela cell with CSB-PA006586LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cell with CSB-PA006586LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA006586LA01HU diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.

Western Blot
Positive WB detected in: K562 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, 293 whole cell lysate, MCF7 whole cell lysate, Rat Brain tissue lysate
All lanes: DDHD2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa