FLOT1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: FLOT1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA008727LA01HU
• Supplier Catalog Number: CSB-PA008727LA01HU
• Alternative Catalog Number: CSB-PA008727LA01HU-100UG, CSB-PA008727LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: FLOT 1 antibody, FLOT1 antibody, FLOT1_HUMAN antibody, Flotillin-1 antibody, Flotillin1 antibody, Integral membrane component of caveolae antibody, Reggie 2 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: O75955
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: FLOT1

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Images

IHC image of CSB-PA008727LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA008727LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.

Western Blot
Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Rat brain tissue, Mouse brain tissue
All lanes: FLOT1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48, 43 kDa
Observed band size: 48 kDa

Immunofluorescence staining of Hela cells with CSB-PA008727LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating FLOT1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA008727LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA008727LA01HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)

Immunoprecipitating FLOT1 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA008727LA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
Lane 2: CSB-PA008727LA01HU (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (20µg)