Fxn Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Fxn Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA009086LA01MO
• Supplier Catalog Number: CSB-PA009086LA01MO
• Alternative Catalog Number: CSB-PA009086LA01MO-100UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: Fxn antibody, FrdaFrataxin antibody, mitochondrial antibody, Fxn antibody, EC 1.16.3.1) [Cleaved into: Frataxin intermediate form, Frataxin mature form] antibody

Product Properties

• Clonality: Polyclonal
• UniProt: O35943
• Buffer: Preservative: 0.02% sodium azide<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: Fxn

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200

Images

IHC image of CSB-PA009086LA01MO diluted at 1:66 and staining in paraffin-embedded human Liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA009086LA01MO diluted at 1:66 and staining in paraffin-embedded human Heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA009086LA01MO diluted at 1:66 and staining in paraffin-embedded rat Liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA009086LA01MO diluted at 1:66 and staining in paraffin-embedded mouse Liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA009086LA01MO diluted at 1:66 and staining in paraffin-embedded mouse Heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), JK whole cell lysate(20µg), Raji whole cell lysate(30µg), A549 whole cell lysate(30µg), Mouse Brain tissue lysate(30µg),Mouse Liver tissue lysate(30µg)
All lanes: Fxn antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 120s