GATM Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA009284ESR1HU
Article Name: GATM Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA009284ESR1HU
Supplier Catalog Number: CSB-PA009284ESR1HU
Alternative Catalog Number: CSB-PA009284ESR1HU-100UL, CSB-PA009284ESR1HU-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human, Mouse
Conjugation: Unconjugated
Alternative Names: AGAT antibody, AT antibody, GATM antibody, GATM_HUMAN antibody, Glycine amidinotransferase antibody, Glycine amidinotransferase, mitochondrial antibody, L-arginine:glycine amidinotransferase antibody, mitochondrial antibody, Transamidinase antibody
Clonality: Polyclonal
UniProt: P50440
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: Antigen Affinity Purified
Form: Liquid
Target: GATM
Application Dilute: Recommended dilution: WB:1:1000-1:3000, IHC:1:200-1:500
IHC image of CSB-PA009284ESR1HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA009284ESR1HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA009284ESR1HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot
Positive WB detected in: THP-1 whole cell lysate(20µg), 293 whole cell lysate(20µg), Mouse Liver tissue lysate(20µg), Mouse Kidney tissue lysate(20µg)
All lanes: GATM antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 45 kDa
Observed band size: 49 kDa
Exposure time: 120s