GPT Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA009864LA01HU
- Images (5)
| Article Name: | GPT Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA009864LA01HU |
| Supplier Catalog Number: | CSB-PA009864LA01HU |
| Alternative Catalog Number: | CSB-PA009864LA01HU-100UG, CSB-PA009864LA01HU-50UG |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, IP, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | AAT1 antibody, Alanine aminotransferase 1 antibody, Alanine aminotransferase antibody, ALAT1_HUMAN antibody, ALT1 antibody, Glutamate pyruvate transaminase 1 antibody, Glutamic alanine transaminase 1 antibody, Glutamic pyruvate transaminase (alanine aminotransferase) antibody, Glutamic pyruvic transaminase 1 antibody, Glutamic--alanine transaminase 1 antibody, Glutamic--pyruvic transaminase 1 antibody, GPT 1 antibody, gpt antibody, GPT1 antibody |
| Clonality: | Polyclonal |
| UniProt: | P24298 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | GPT |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000 |
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IHC image of CSB-PA009864LA01HU diluted at 1:350 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunofluorescent analysis of HepG2 cells using CSB-PA009864LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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IHC image of CSB-PA009864LA01HU diluted at 1:350 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating GPT in Mouse liver tissue Lane 1: Rabbit control IgG instead of CSB-PA009864LA01HU in Mouse liver tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA009864LA01HU (8µg) + Mouse liver tissue (500µg) Lane 3: Mouse liver tissue (10µg) |
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Western Blot Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue, Mouse liver tissue All lanes: GPT antibody at 4.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa |
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