H2AFZ (Ab-4) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: H2AFZ (Ab-4) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA010100OA04NACHU
• Supplier Catalog Number: CSB-PA010100OA04nacHU
• Alternative Catalog Number: CSB-PA010100OA04NACHU-100UL, CSB-PA010100OA04NACHU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: H2A histone family member Z antibody, H2A.z antibody, H2A/z antibody, H2afz antibody, H2AZ antibody, H2AZ_HUMAN antibody, Histone H2A.Z antibody, MGC117173 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P0C0S5
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: H2AFZ

Application Notes

• Application Dilute: Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:10-1:100, IP:1:200-1:2000

Images

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFZ (CSB-PA010100OA04nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Immunofluorescence staining of Hela cells with CSB-PA010100OA04nacHU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA010100OA04nacHU diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA010100OA04nacHU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: K562 whole cell lysate, Mouse spleen tissue
All lanes: H2AFZ antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa

Immunoprecipitating H2AFZ in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA010100OA04nacHU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010100OA04nacHU (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)