HDAC3 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HDAC3 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA010239LA01HU
• Supplier Catalog Number: CSB-PA010239LA01HU
• Alternative Catalog Number: CSB-PA010239LA01HU-100UG, CSB-PA010239LA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: HD3 antibody, HDAC 3 antibody, HDAC3 antibody, HDAC3_HUMAN antibody, Histone deacetylase 3 antibody, RPD3 2 antibody, RPD3 antibody, RPD3-2 antibody, SMAP45 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: O15379
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: HDAC3

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

Images

Chromatin Immunoprecipitation Hela(1.2*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HDAC3 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP010239HU) against the P21 promoter.

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA010239LA01HU at dilution of 1:100

Western blot
All lanes: HDAC3 antibody at 2µg/ml + Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49, 50 kDa
Observed band size: 49 kDa

Western Blot
Positive WB detected in: Mouse heart tissue
All lanes: HDAC3 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 50 kDa
Observed band size: 49 kDa

Immunofluorescent analysis of Hela cells using CSB-PA010239LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)