2-hydroxyisobutyryl-HIST1H1C (K158) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: 2-hydroxyisobutyryl-HIST1H1C (K158) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA010378OA158HIBHU
• Supplier Catalog Number: CSB-PA010378OA158hibHU
• Alternative Catalog Number: CSB-PA010378OA158HIBHU-100UL, CSB-PA010378OA158HIBHU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, ICC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: H1 histone family member 2 antibody, H1.a antibody, H12_HUMAN antibody, H1F2 antibody, H1s-1 antibody, HIST1H1C antibody, Histone 1 H1c antibody, Histone cluster 1 H1c antibody, Histone H1.2 antibody, Histone H1c antibody, Histone H1d antibody, Histone H1s-1 antibody, MGC3992 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P16403
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: HIST1H1C

Application Notes

• Application Dilute: Recommended dilution: WB:1:100-1:1000, ICC:1:10-1:100

Images

Western Blot
Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
All lanes: HIST1H1C antibody at 1:100
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa

Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1C (CSB-PA010378OA158hibHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Immunocytochemistry analysis of CSB-PA010378OA158hibHU diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.