HIST1H1E (Ab-25) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HIST1H1E (Ab-25) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA010380PA25NME1HU
• Supplier Catalog Number: CSB-PA010380PA25nme1HU
• Alternative Catalog Number: CSB-PA010380PA25NME1HU-100UL, CSB-PA010380PA25NME1HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: H1 histone family member 4 antibody, H1.4 antibody, H14_HUMAN antibody, H1E antibody, H1F4 antibody, Hist1h1e antibody, Histone 1 H1e antibody, Histone cluster 1 H1e antibody, Histone H1 antibody, Histone H1.4 antibody, Histone H1B antibody, MGC116819 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P10412
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: HIST1H1E

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200

Images

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1E (CSB-PA010380PA25nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Immunofluorescent analysis of Hela cells using CSB-PA010380PA25nme1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010380PA25nme1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA010380PA25nme1HU at dilution of 1:100

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, MCF-7 whole cell lysate, LO2 whole cell lysate
All lanes: HIST1H1E antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa