HIST1H2AG (Ab-36) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HIST1H2AG (Ab-36) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA010389PA36NACHU
• Supplier Catalog Number: CSB-PA010389PA36nacHU
• Alternative Catalog Number: CSB-PA010389PA36NACHU-100UL, CSB-PA010389PA36NACHU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: H2AC11 antibody, H2AFP antibody, HIST1H2AG, antibody, H2AC13 antibody, H2AFC antibody, HIST1H2AI, antibody, H2AC15 antibody, H2AFD antibody, HIST1H2AK, antibody, H2AC16 antibody, H2AFI antibody, HIST1H2AL, antibody, H2AC17 antibody, H2AFN antibody, HIST1H2AMHistone H2A type 1 antibody, H2A.1 antibody, Histone H2A/ptl antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P0C0S8
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: HIST1H2AG

Application Notes

• Application Dilute: Recommended dilution: WB:1:200-1:2000, IHC:1:20-1:200, IF:1:50-1:200

Images

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2AG (CSB-PA010389PA36nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Immunohistochemistry of paraffin-embedded human glioma using CSB-PA010389PA36nacHU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA010389PA36nacHU at dilution of 1:100

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: HIST1H2AG antibody at 1.25µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa

Immunofluorescence staining of Hela cells with CSB-PA010389PA36nacHU at 1:62, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).