Acetyl-HIST1H2BC (K20) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Acetyl-HIST1H2BC (K20) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA010403OA20ACHU
• Supplier Catalog Number: CSB-PA010403OA20acHU
• Alternative Catalog Number: CSB-PA010403OA20ACHU-100UL, CSB-PA010403OA20ACHU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, ICC, IF, IP, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: H2BC4 antibody, H2BFL antibody, HIST1H2BC, antibody, H2BC6 antibody, H2BFH antibody, HIST1H2BE, antibody, H2BC7 antibody, H2BFG antibody, HIST1H2BF, antibody, H2BC8 antibody, H2BFA antibody, HIST1H2BG, antibody, H2BC10 antibody, H2BFK antibody, HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody, Histone H2B.1 A antibody, Histone H2B.a antibody, H2B/a antibody, Histone H2B.g antibody, H2B/g antibody, Histone H2B.h antibody, H2B/h antibody, Histone H2B.k antibody, H2B/k antibody, Histone H2B.l antibody, H2B/l antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P62807
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: HIST1H2BC

Application Notes

• Application Dilute: Recommended dilution: WB:1:100-1:1000, ICC:1:10-1:100, IF:1:1-1:10, IP:1:200-1:2000

Images

Immunocytochemistry analysis of CSB-PA010403OA20acHU diluted at 1:15 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Detected samples: 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
All lanes: HIST1H2BC antibody at 1:100
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa

Chromatin Immunoprecipitation Hela (106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (CSB-PA010403OA20acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010403OA20acHU at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating HIST1H2BC in A549 whole cell lysate (treated with 30mM sodium butyrate for 4h)
Lane 1: Rabbit control IgG instead of CSB-PA010403OA20acHU in A549 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010403OA20acHU (5µg) + A549 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg)
Lane 3: A549 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)