Acetyl-HIST1H3A (K27) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Acetyl-HIST1H3A (K27) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA010418OA27ACHU
• Supplier Catalog Number: CSB-PA010418OA27acHU
• Alternative Catalog Number: CSB-PA010418OA27ACHU-100UL, CSB-PA010418OA27ACHU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, ICC, IF, IP, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: H3 histone family member E pseudogene antibody, H3 histone family, member A antibody, H3/A antibody, H31_HUMAN antibody, H3F3 antibody, H3FA antibody, Hist1h3a antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST3H3 antibody, histone 1, H3a antibody, Histone cluster 1, H3a antibody, Histone H3 3 pseudogene antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P68431
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: HIST1H3A

Application Notes

• Application Dilute: Recommended dilution: WB:1:100-1:1000, ICC:1:10-1:100, IF:1:1-1:10, IP:1:200-1:2000

Images

Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418OA27acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Immunocytochemistry analysis of CSB-PA010418OA27acHU diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010418OA27acHU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Detected samples: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
All lanes: HIST1H3A antibody at 1:100
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa

Immunoprecipitating HIST1H3A in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010418OA27acHU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010418OA27acHU (5µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)