HIST1H4A (Ab-8) Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HIST1H4A (Ab-8) Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA010429PA08NACHU
• Supplier Catalog Number: CSB-PA010429PA08nacHU
• Alternative Catalog Number: CSB-PA010429PA08NACHU-100UL, CSB-PA010429PA08NACHU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: dJ160A22.1 antibody, dJ160A22.2 antibody, dJ221C16.1 antibody, dJ221C16.9 antibody, FO108 antibody, H4 antibody, H4.k antibody, H4/a antibody, H4/b antibody, H4/c antibody, H4/d antibody, H4/e antibody, H4/g antibody, H4/h antibody, H4/I antibody, H4/j antibody, H4/k antibody, H4/m antibody, H4/n antibody, H4/p antibody, H4_HUMAN antibody, H4F2 antibody, H4F2iii antibody, H4F2iv antibody, H4FA antibody, H4FB antibody, H4FC antibody, H4FD antibody, H4FE antibody, H4FG antibody, H4FH antibody, H4FI antibody, H4FJ antibody, H4FK antibody, H4FM antibody, H4FN antibody, H4M antibody, HIST1H4A antibody, HIST1H4B antibody, HIST1H4C antibody, HIST1H4D antibody, HIST1H4E antibody, HIST1H4F antibody, HIST1H4H antibody, HIST1H4I antibody, HIST1H4J antibody, HIST1H4K antibody, HIST1H4L antibody, HIST2H4 antibody, HIST2H4A antibody, Hist4h4 antibody, Histone 1 H4a antibody, Histone 1 H4b antibody, Histone 1 H4c antibody, Histone 1 H4d antibody, Histone 1 H4e antibody, Histone 1 H4f antibody, Histone 1 H4h antibody, Histone 1 H4i antibody, Histone 1 H4j antibody, Histone 1 H4k antibody, Histone 1 H4l antibody, Histone 2 H4a antibody, histone 4 H4 antibody, Histone H4 antibody, MGC24116 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P62805
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: Antigen Affinity Purified
• Form: Liquid
• Target: HIST1H4A

Application Notes

• Application Dilute: Recommended dilution: WB:1:100-1:1000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Images

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H4A (CSB-PA010429PA08nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.

Immunofluorescent analysis of Hela cells using CSB-PA010429PA08nacHU at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010429PA08nacHU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010429PA08nacHU at dilution of 1:100

Western Blot
Positive WB detected in: Rat liver tissue
All lanes: HIST1H4A antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa

Immunoprecipitating HIST1H4A in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010429PA08nacHU in NIH/3T3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010429PA08nacHU (5µg) + NIH/3T3 whole cell lysate (500µg)
Lane 3: NIH/3T3 whole cell lysate (20µg)