SLC16A3 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: SLC16A3 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA021410LA01HU
• Supplier Catalog Number: CSB-PA021410LA01HU
• Alternative Catalog Number: CSB-PA021410LA01HU-100UL, CSB-PA021410LA01HU-50UL
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: MCT 4 antibody, MCT3 antibody, MCT4 antibody, Monocarboxylate transporter 3 antibody, Monocarboxylate transporter 4 antibody, MOT4_HUMAN antibody, SLC16A3 antibody, Solute carrier family 16 member 3 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: O15427
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: SLC16A3

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:20-1:200

Images

Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate

Immunofluorescence staining of HepG2 cell with CSB-PA021410LA01HU at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA021410LA01HU diluted at 1:300 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: PC-3 whole cell lysate, MCF7 whole cell lysate
All lanes:SLC16A3 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size:50 kDa
Observed band size:50 kDa