SMAD3 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
CSB-PA021788ESR2HU
- Images (6)
| Article Name: | SMAD3 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | CSB-PA021788ESR2HU |
| Supplier Catalog Number: | CSB-PA021788ESR2HU |
| Alternative Catalog Number: | CSB-PA021788ESR2HU-100UL, CSB-PA021788ESR2HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC, IP, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | DKFZP586N0721 antibody, DKFZp686J10186 antibody, hMAD 3 antibody, hMAD-3 antibody, hSMAD3 antibody, HSPC193 antibody, HST17436 antibody, JV15 2 antibody, JV15-2 antibody, JV152 antibody, LDS1C antibody, LDS3 antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody, MAD homolog 3 antibody, Mad homolog JV15 2 antibody, Mad protein homolog antibody, MAD, mothers against decapentaplegic homolog 3 antibody, Mad3 antibody, MADH 3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody, SMA and MAD related protein 3 antibody, SMAD 3 antibody, SMAD antibody, SMAD family member 3 antibody, SMAD, mothers against DPP homolog 3 antibody, Smad3 antibody, SMAD3_HUMAN antibody |
| Clonality: | Polyclonal |
| UniProt: | P84022 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purity: | Antigen Affinity Purified |
| Form: | Liquid |
| Target: | SMAD3 |
| Application Dilute: | Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:500, IF:1:50-1:200, IP:1:200-1:2000 |
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA021788ESR2HU at dilution of 1:100 |
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA021788ESR2HU at dilution of 1:100 |
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Immunofluorescence staining of A549 cells with CSB-PA021788ESR2HU at 1:129, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-PA021788ESR2HU diluted at 1:388 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
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Immunoprecipitating SMAD3 in Jurkat whole cell lysate Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA021788ESR2HU in Jurkat whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA021788ESR2HU (4ug) + Jurkat whole cell lysate (500ug) Lane 3: Jurkat whole cell lysate (20ug) |
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Western blot All lanes: SMAD3 antibody at 8ug/ml Lane 1: Jurkat whole cell lysate Lane 2: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 49, 44, 36, 26 kDa Observed band size: 49 kDa |
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