SYNJ2BP Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA023018LA01HU
Article Name: SYNJ2BP Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA023018LA01HU
Supplier Catalog Number: CSB-PA023018LA01HU
Alternative Catalog Number: CSB-PA023018LA01HU-100UG, CSB-PA023018LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, IP, WB
Species Reactivity: Human, Rat
Conjugation: Unconjugated
Alternative Names: SYNJ2BP antibody, OMP25 antibody, Synaptojanin-2-binding protein antibody, Mitochondrial outer membrane protein 25 antibody
Clonality: Polyclonal
UniProt: P57105
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: >95%, Protein G purified
Form: Liquid
Target: SYNJ2BP
Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA023018LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA023018LA01HU at dilution of 1:100
Western blot
All lanes: SYNJ2BP antibody at 3µg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: U251 whole cell lysate
Lane 3: Caco-2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa

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Western Blot
Positive WB detected in: Rat kidney tissue
All lanes: SYNJ2BP antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Immunohistochemistry analysis of human liver cancer using CSB-PA023018LA01HU at dilution of 1:100
Immunoprecipitating SYNJ2BP in Rat kidney tissue
Lane 1: Rabbit control IgG instead of CSB-PA023018LA01HU in Rat kidney tissue. For western blotting, a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA023018LA01HU (6µg) + Rat kidney tissue (500µg)
Lane 3: Rat kidney tissue (20µg)