TMEM41A Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA023837LA01HU
Article Name: TMEM41A Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA023837LA01HU
Supplier Catalog Number: CSB-PA023837LA01HU
Alternative Catalog Number: CSB-PA023837LA01HU-100UG, CSB-PA023837LA01HU-50UG
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, WB
Species Reactivity: Human
Conjugation: Unconjugated
Alternative Names: 2900010K02Rik antibody, TM41A_HUMAN antibody, Tmem41a antibody, Transmembrane protein 41A antibody, UNQ168/PRO194 antibody
Clonality: Polyclonal
UniProt: Q96HV5
Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purity: Antigen Affinity Purified
Form: Liquid
Target: TMEM41A
Application Dilute: Recommended dilution: WB:1:500-1:2000, IF:1:1:20-1:200
Immunohistochemistry of paraffin-em
Immunofluorescence staining of HepG2 cell with CSB-PA023837LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of PC-3 cell with CSB-PA023837LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of PC-3 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: A431 whole cell lysate(30µg), K562 whole cell lysate(30µg), 293 whole cell lysate(30µg), PC-3 whole cell lysate(30µg)
All lanes: TMEM41A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 120s