TPST1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA024132LA01HU
Article Name: TPST1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA024132LA01HU
Supplier Catalog Number: CSB-PA024132LA01HU
Alternative Catalog Number: CSB-PA024132LA01HU-100UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Alternative Names: Protein tyrosine sulfotransferase 1 antibody, Protein-tyrosine sulfotransferase 1 antibody, TPST-1 antibody, TPST1 antibody, TPST1_HUMAN antibody, Tyrosylprotein sulfotransferase 1 antibody
Clonality: Polyclonal
UniProt: O60507
Buffer: Preservative: 0.02% sodium azide<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purity: Antigen Affinity Purified
Form: Liquid
Target: TPST1
Application Dilute: Recommended dilution: WB:1:1000-1:3000, IHC:1:50-1:200
IHC image of CSB-PA024132LA01HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA024132LA01HU diluted at 1:50 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in: Mouse Skeletal Muscle tissue lysate(20µg),Rat Skeletal Muscle tissue lysate(20µg)
All lanes: TPST1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 42.2 kDa
Observed band size: 42 kDa
Exposure time: 120s