YWHAE Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: YWHAE Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA026287DA01HU
• Supplier Catalog Number: CSB-PA026287DA01HU
• Alternative Catalog Number: CSB-PA026287DA01HU-100UG, CSB-PA026287DA01HU-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: 14 3 3 E antibody, 14 3 3 epsilon antibody, 14 3 3E antibody, 14-3-3 protein epsilon antibody, 14-3-3E antibody, 1433E_HUMAN antibody, Epididymis luminal protein 2 antibody, FLJ45465 antibody, FLJ53559 antibody, HEL2 antibody, KCIP 1 antibody, KCIP1 antibody, MDCR antibody, MDS antibody, Mitochondrial import stimulation factor L subunit antibody, Protein kinase C inhibitor protein1 antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon antibody, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, epsilon polypeptide antibody, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide antibody, YWHAE antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P62258
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: YWHAE

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:1000-1:2000, IF:1:200-1:500

Images

Immunofluorescence staining of HepG2 cells with CSB-PA026287DA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA026287DA01HU diluted at 1:1000 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA026287DA01HU diluted at 1:1000 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: A375 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, Mouse brain tissue
All lanes: YWHAE antibody at 3.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30, 27 kDa
Observed band size: 30 kDa

IHC image of CSB-PA026287DA01HU diluted at 1:1000 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, Mouse heart tissue
All lanes: YWHAE antibody at 3.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30, 27 kDa
Observed band size: 30, 27 kDa