LGALS9 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: LGALS9 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA02649A0RB
• Supplier Catalog Number: CSB-PA02649A0Rb
• Alternative Catalog Number: CSB-PA02649A0RB-100UG, CSB-PA02649A0RB-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: 36 kDa beta-galactoside-binding lectin antibody, Ecalectin antibody, Gal-9 antibody, galectin 9 antibody, Galectin-9 antibody, galectin9 antibody, HOM HD 21 antibody, HOMHD21 antibody, HUAT antibody, Lectin galactoside binding soluble 9 antibody, LEG9_HUMAN antibody, LGAL S9 antibody, LGALS 9 antibody, Lgals9 antibody, LGALS9A antibody, MGC117375 antibody, MGC125973 antibody, MGC125974 antibody, Tumor antigen HOM-HD-21 antibody, UAT antibody, Urate transporter/channel antibody, Urate transporter/channel protein antibody

Product Properties

• Clonality: Polyclonal
• UniProt: O00182
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: LGALS9

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Images

Immunofluorescence staining of NIH/3T3 cells with CSB-PA02649A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA02649A0Rb diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA02649A0Rb diluted at 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Mouse heart tissue
All lanes: LGALS9 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40, 36, 35, 27, 26, 39 kDa
Observed band size: 40 kDa

IHC image of CSB-PA02649A0Rb diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.