IFNA2 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: CSB-PA06504A0RB
Article Name: IFNA2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: CSB-PA06504A0RB
Supplier Catalog Number: CSB-PA06504A0Rb
Alternative Catalog Number: CSB-PA06504A0RB-100UL, CSB-PA06504A0RB-50UL
Manufacturer: Cusabio
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human, Mouse, Rat
Conjugation: Unconjugated
Alternative Names: Alpha 2a interferon antibody, IFN alpha 2b antibody, IFN-alpha-2 antibody, IFNA antibody, Ifna2 antibody, IFNA2_HUMAN antibody, INFA2 antibody, Interferon alpha 2 antibody, Interferon alpha A antibody, Interferon alpha-2 antibody, Interferon alpha-A antibody, LeIF A antibody, LeIFA antibody
Clonality: Polyclonal
UniProt: P01563
Buffer: Preservative: 0.05% NaN3<br /> Constituents: PBS containing 40% glycerol,pH7.4
Purity: Antigen Affinity Purified
Form: Liquid
Target: IFNA2
Application Dilute: Recommended dilution: WB:1:1000-1:5000 , IHC:1:100-1:300
Immunohistochemistry
IHC image of CSB-PA06504A0Rb diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-PA06504A0Rb diluted at 1:200 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-PA06504A0Rb diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-PA06504A0Rb diluted at 1:200 and staining in paraffin-embedded Mouse kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot
Positive WB detected in: U-937 whole cell lysate, SP2/0 whole cell lysate, Mouse Kidney tissue lysate, Rat Liver tissue lysate
All lanes: IFNA2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 18-22 kDa