MIF Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: MIF Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA06867A0RB
• Supplier Catalog Number: CSB-PA06867A0Rb
• Alternative Catalog Number: CSB-PA06867A0RB-100UG, CSB-PA06867A0RB-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Conjugation: Unconjugated
• Alternative Names: GIF antibody, GLIF antibody, Glycosylation inhibiting factor antibody, Glycosylation-inhibiting factor antibody, L-dopachrome isomerase antibody, L-dopachrome tautomerase antibody, Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody, Macrophage migration inhibitory factor antibody, MIF antibody, MIF protein antibody, MIF_HUMAN antibody, MMIF antibody, Phenylpyruvate tautomerase antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P14174
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: MIF

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200

Images

Immunofluorescence staining of Hela cells with CSB-PA06867A0Rb at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA06867A0Rb diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA06867A0Rb diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA06867A0Rb diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA06867A0Rb diluted at 1:400 and staining in paraffin-embeddedhuman testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.

Western Blot
Positive WB detected in: Jurkat whole cell lysate, Rat kidney tissue, Mouse kidney tissue
All lanes: MIF antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa