TUBA4A Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: TUBA4A Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA09499A0RB
• Supplier Catalog Number: CSB-PA09499A0Rb
• Alternative Catalog Number: CSB-PA09499A0RB-100UG, CSB-PA09499A0RB-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, IP, WB
• Species Reactivity: Human, Mouse
• Conjugation: Unconjugated
• Alternative Names: Alpha-tubulin 1 antibody, ALS22 antibody, B ALPHA 1 antibody, bA408E5.3 antibody, H2 ALPHA antibody, Hum a tub1 antibody, Hum a tub2 antibody, LIS3 antibody, MGC171407 antibody, MGC55332 antibody, TBA4A_HUMAN antibody, Testis-specific alpha-tubulin antibody, TUBA1 antibody, TUBA1A antibody, tuba1l antibody, Tuba4a antibody, Tubulin alpha 1 chain antibody, Tubulin alpha antibody, Tubulin alpha-1 chain antibody, tubulin alpha-1B chain antibody, Tubulin alpha-4A chain antibody, Tubulin H2-alpha antibody, Tubulin, alpha 1 (testis specific) antibody, tubulin, alpha 1, like antibody, Tubulin, alpha 4a antibody, Tubulin, alpha, testis-specific antibody, Tubulin, alpha-1 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P68366
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: TUBA4A

Application Notes

• Application Dilute: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Images

Immunofluorescent analysis of HepG2 cells using CSB-PA09499A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA09499A0Rb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA09499A0Rb at dilution of 1:100

Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse brain tissue
All lanes: TUBA4A antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 49 kDa
Observed band size: 50, 24 kDa

Immunoprecipitating TUBA4A in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA09499A0Rb in K562 whole cell lysate.For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA09499A0Rb (4ug) + K562 whole cell lysate (500ug)
Lane 3: K562 whole cell lysate (20ug)