NFKB1 Antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: NFKB1 Antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: CSB-PA10354A0RB
• Supplier Catalog Number: CSB-PA10354A0Rb
• Alternative Catalog Number: CSB-PA10354A0RB-100UG, CSB-PA10354A0RB-50UG
• Manufacturer: Cusabio
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ELISA, IF, IHC, WB
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: DKFZp686C01211 antibody, DNA binding factor KBF1 antibody, DNA binding factor KBF1 EBP1 antibody, DNA-binding factor KBF1 antibody, EBP 1 antibody, EBP-1 antibody, EBP1 antibody, KBF1 antibody, MGC54151 antibody, nf b antibody, NF kappa B antibody, NF kappaB antibody, NF kappabeta antibody, NF kB1 antibody, NF-kappaB antibody, NFkappaB antibody, NFKB 1 antibody, NFKB p105 antibody, NFKB p50 antibody, NFKB-p105 antibody, Nfkb1 antibody, NFKB1_HUMAN antibody, Nuclear factor kappa B DNA binding subunit antibody, Nuclear factor kappa-B antibody, Nuclear factor kappa-B, subunit 1 antibody, Nuclear factor NF kappa B p105 subunit antibody, Nuclear factor NF kappa B p50 subunit antibody, Nuclear factor NF-kappa-B p50 subunit antibody, Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody, Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody, p105 antibody, p50 antibody, p84/NF-kappa-B1 p98 antibody, Transcription factor NFKB1 antibody

Product Properties

• Clonality: Polyclonal
• UniProt: P19838
• Buffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Purity: >95%, Protein G purified
• Form: Liquid
• Target: NFKB1

Application Notes

• Application Dilute: Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000, IF:1:200-1:500

Images

Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-NFKB1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP10354HU) against the IkB promoter.

Immunofluorescence staining of MCF-7 cells with CSB-PA10354A0Rb at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°,C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA10354A0Rb diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA10354A0Rb diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Raji whole cell lysate
All lanes: NFKB1 antibody at 3.75µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 106, 86 kDa
Observed band size: 106 kDa

IHC image of CSB-PA10354A0Rb diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°,C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated ABC system.